Induction and evaluation of colchitetraploids of two species of Tinospora Miers, 1851

By

Thakur, Rakesh
Rajpal, Vijay Rani
Rama Rao, Satyawada
Singh, Apekshita
Joshi, Lata
Kaushal, Pankaj
Raina, Soom

Type

Article

Date of Publication

2020

Original Publication

Comparative cytogenetics : international journal of plant & animal cytogenetics, karyosystematics and molecular systematics

Volume

14

Series / Issue

Issue: 2

Pages

211--229

Subjects

colchicine treatment , Cytogenetics , flow cytometry , Morphology , polyploidy , Tinospora cordifolia , Tinospora sinensis

Contributed by

Pensoft Publishers

Language

English

Published by

St. Petersburg, Russia [Published] with technical support of Zoological Institute RAS [2007]-

Identifiers

DOI: https://doi.org/10.3897/CompCytogen.v14i2.33394

 

Abstract

Autotetraploidy, both natural and/or induced, has potential for genetic improvement of various crop species including that of medicinal importance.Tinospora cordifolia(Willdenow, 1806) Miers, 1851 ex Hooker et Thomson, 1855 andT. sinensis(Loureiro, 1790) Merrill, 1934 are two diploid species, which are dioecious, deciduous and climbing shrubs with high medicinal importance. Among the three methods used for induction of polyploidy by colchicine treatment, it was cotton swab method which successfully induced the polyploidy in both species. The morphological and cytogenetical features of the synthetic tetraploids were compared with their diploid counterparts. The tetraploids were morphologically distinct from diploid plants. They exhibited larger organs, such as stem, leaves, inflorescence, fruits, flowers and seeds. The tetraploids were characterized by the presence of low quadrivalent frequency and high bivalent average. Unequal distribution of chromosomes at anaphase I was found in 60% cells. The present study provides important information on the superiority of autotetraploids as compared to diploid counterparts in both species.