Spiders of the genusClubionaLatreille, 1804 from Xishuangbanna, Yunnan Province, China are studied. A total of 47 species is reported and illustrated, including 14 new species and two new synonyms. Twelve of the new species belong to four species groups:C. dengpaoYu & Li,sp. nov.,C. subdidentataYu & Li,sp. nov.,C. tixingYu & Li,sp. nov.,C. xiaociYu & Li,sp. nov.,C. xiaokongYu & Li,sp. nov.,C. yejieiYu & Li,sp. nov.,C. zhaoiYu & Li,sp. nov.andC. zhigangiYu & Li,sp. nov.from theC. corticalisgroup;C. miiYu & Li,sp. nov.andC. subtongiYu & Li,sp. nov.from theC. ternatensisgroup;C. bannaYu & Li,sp. nov.from theC. filicatagroup; andC. menglunYu & Li,sp. nov.from theC. trivialisgroup. The remaining two new species,C. shuangsiYu & Li,sp. nov.andC. wangchengiYu & Li,sp. nov., are not readily assignable to any of the existing species groups. The female ofC. cochlearisYu & Li, 2019, the female ofC. tianeYu & Li, 2019, the female ofC. bicornisYu & Li, 2019, the male ofC. lalaJäger & Dankittipakul, 2010 and the true female ofC. suthepicaDankittipakul, 2008 are described for the first time. Two new synonyms are:C. vukomiJäger & Dankittipakul, 2010syn. nov. =C. circulataZhang & Yin, 1998;C. melanotheleThorell, 1895syn. nov. =Clubiona melanostictaThorell, 1890. A checklist ofClubionaspecies from Xishuangbanna is provided. The DNA barcodes of almost all of the species were obtained for species delimitation, matching of sexes and future use.