Molecular diagnostic based on 18S rDNA and supplemental taxonomic data of the cnidarian coelozoic Ceratomyxa (Cnidaria, Myxosporea) and comments on the intraspecific morphological variation

By

B. Sousa, Fabricio
Milanin, Tiago
Morandini, André Carrara
L. Espinoza, Luis
Flores-Gonzales, Anai
Gomes, Ana Lúcia Silva
A. Matoso, Daniele
Mathews Delgado, Patrick

Type

Article

Date of Publication

2021

Original Publication

Zoosystematics and evolution

Volume

97

Pages

307--314

Subjects

Brazil , ceratomyxid , morphological plasticity , Ornamental fish , parasitic cnidarian

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia Pensoft

Identifiers

DOI: https://doi.org/10.3897/zse.97.64769

 

Abstract

Ceratomyxa amazonensis is a cnidarian myxosporean originally described with strongly arcuate crescent-shaped myxospores, absence of vegetative stages and infecting Symphysodon discus, an important Amazonian ornamental fish in the aquarium industry. As part of a long-term investigation concerning myxosporeans that infect discus fish Symphysodon spp. from different rivers of the Amazon Basin, thirty specimens of S. discus collected from Unini River were examined. Plasmodial vegetative stages therefrom were found freely floating in the bile of gall bladders from eighteen fish. Mature myxospores were slightly crescent-shaped, measuring 4.72 ± 0.1 (4.52–4.81) μm in length, 24.2 ± 0.4 (23.9–25.3) μm in thickness with polar capsules 2.31 ± 0.1 (2.29–2.33) μm in length and 2.15 ± 0.1 (2.13–2.17) μm in width. Strong morphological differences were observed between the newly isolated myxospores obtained and the previously described C. amazonensis; however, molecular assessment, based on 18S rDNA, revealed a high similarity (99.91%), with only a single nucleotide base change. This study provides new data, expanding the original description of the species with a discussion on differences in myxospore-morphology in the context of intraspecific morphological plasticity.