Two new Inosperma (Inocybaceae) species with unexpected muscarine contents from tropical China

By

Deng, Lun-Sha
Kang, Rui
Zeng, Nian-Kai
Yu, Wen-Jie
Chang, Cheng
Xu, Fei
Deng, Wang-Qiu
Qi, Liang-Liang
Zhou, Yu-Ling
Fan, Yuguang

Type

Article

Date of Publication

2021

Original Publication

Journal of Hymenoptera research

Volume

85

Pages

87--108

Subjects

Agaricales , muscarine , new species , Phylogeny , taxonomy

Contributed by

Pensoft Publishers

Language

English

Published by

Washington, D.C International Society of Hymenopterists

Identifiers

DOI: https://doi.org/10.3897/mycokeys.85.71957

 

Abstract

An accurate identification of poisonous mushrooms and the confirmation of the toxins involved are both of great importance in the treatment of mushroom poisoning incidents. In recent years, cases of mushroom poisoning by Inosperma spp. have been repeatedly reported from tropical Asia. It is urgent to know the real species diversity of Inosperma in this region. In the present study, we proposed two new Inosperma species from tropical Asia, namely I. muscarium and I. hainanense. They were described based on morphology and multilocus phylogeny. Detailed descriptions, color photographs and the discussion with other closely related species of the two new taxa were provided. In addition, a comprehensive muscarine determination of these two new species using ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) approach has been performed. Results showed that these two species were muscarine positive, with a content of 16.03 ± 1.23 g/kg in I. muscarium and a content of 11.87 ± 3.02 g/kg in I. hainanense, much higher than the known species I. virosum. Recovery of muscarine ranged from 93.45% to 97.25%, and the average recovery is 95.56%.