Comparative chromosome studies in species of subtribe Orchidinae (Orchidaceae)

By

Turco, Alessio
Albano, Antonella
Medagli, Pietro
Wagensommer, Robert Philipp
D'Emerico, Saverio

Type

Article

Date of Publication

2021

Original Publication

Comparative cytogenetics : international journal of plant & animal cytogenetics, karyosystematics and molecular systematics

Volume

15

Series / Issue

Issue: 4

Pages

507--525

Subjects

Anacamptis , C-banding , cytotaxonomy , Fish , Himantoglossum , karyotype , Ophrys , Serapias

Contributed by

Pensoft Publishers

Language

English

Published by

St. Petersburg, Russia [Published] with technical support of Zoological Institute RAS [2007]-

Identifiers

DOI: https://doi.org/10.3897/compcytogen.v15.i4.75990

 

Abstract

In our study, FISH mapping using 18S-5.8S-25S rDNA and 5S rDNA sequences was performed for the first time onOphrys tenthrediniferaWilldenow, 1805,Serapias vomeracea(Burman f., 1770) Briquet, 1910 andHimantoglossum hircinum(Linnaeus, 1753) Sprengel, 1826. A detailed study was also performed onO. tenthrediniferausing Giemsa-staining, silver-staining, CMA fluorescence banding and fluorescencein situhybridisation (FISH) with rDNA probes. We analysed two subspecies, i.e. O. tenthredinifera subsp. neglecta (Parlatore, 1860) E.G. Camus, 1908 and O. tenthredinifera subsp. grandiflora (Tenore, 1819) Kreutz, 2004 by the traditional Feulgen method and constructed the karyotype. The cytotaxonomic implications for both taxa are also discussed. InHimantoglossum hircinum, FISH and silver staining highlighted differences in the number of two rDNA families (35S and 5S) with respect toBarlia robertiana(Loiseleur-Deslongchamps, 1807) Greuter, 1967. In addition, fluorescencein situhybridisation was also applied to diploid (2n= 2x= 36) and triploid (2n= 3x= 54)Anacamptis morio(Linnaeus, 1753) R.M. Bateman, Pridgeon et M.W. Chase, 1997. As far as we are aware, this is the first case of autotriploidy observed inA. morio.