Karyotype and genome size variation in white-flowered Eranthis sect. Shibateranthis (Ranunculaceae)

By

Mitrenina, Elizaveta
Erst, Andrey
Peruzzi, Lorenzo
Skaptsov, Mikhail
Ikeda, Hiroshi
Nikulin, Vyacheslav
Wang, Wei

Type

Article

Date of Publication

2021

Original Publication

PhytoKeys

Volume

187

Pages

207--227

Subjects

Asia , Chromosomes , Eranthis , genome size , karyotype , Ranunculaceae

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers [2010]-

Identifiers

DOI: https://doi.org/10.3897/phytokeys.187.75715

 

Abstract

Comparative karyomorphological analyses of six out of the eight white-flowered species of Eranthis sect. Shibateranthis have been carried out. All studied specimens ofE. byunsanensis,E. lobulata,E. pinnatifida, andE. stellatahad a somatic chromosome number 2n= 16 with basic chromosome numberx= 8. On the contrary,E. tanhoensisandE. sibiricahad a basic chromosome numberx= 7. The specimens ofE. tanhoensiswere diploid with 2n= 14, while the specimens ofE. sibiricawere polyploid with 2n= 42. Monoploid chromosome sets of the investigated diploid species had 4–5 metacentric chromosomes and 2–4 submetacentric/subtelocentric/acrocentric chromosomes. The highest level of interchromosomal asymmetry, estimated via CVCL, was found inE. byunsanensisandE. pinnatifida. The highest levels of intrachromosomal asymmetry (MCA) and heterogeneity in centromere position (CVCI) were found inE. lobulataandE. byunsanensis, whileE. sibiricahad the most symmetric karyotype. A multivariate PCoA analysis of basic karyotype parameters (2n,x, THL, CVCL, MCA, and CVCI) highlighted no overlap among species accessions, which was also confirmed by LDA. The average absolute monoploid DNA content (1Cx) of the 23 investigated samples of sixEranthisspecies varied from 9.26 ± 0.25 pg inE. sibiricato 15.93 ± 0.32 pg inE. stellata. Overall karyological affinity was highlighted betweenE. lobulataandE. stellata, on one side, and betweenE. byunsanensisandE. pinnatifida, on the other side. Interestingly, there was no significant correlation between total haploid (monoploid) chromosome length (THL) and 1Cxvalues in these species.