Complete mitochondrial genomes of the slugs Deroceras laeve (Agriolimacidae) and Ambigolimax valentianus (Limacidae) provide insights into the phylogeny of Stylommatophora (Mollusca, Gastropoda)

By

Zhao, Te
Song, Nan
Lin, Xingyu
Zhang, Ying

Type

Article

Date of Publication

2023

Original Publication

ZooKeys

Volume

1173

Pages

43--59

Subjects

gene rearrangement , Limacoidea , Mitogenome , next generation sequencing , Phylogeny

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.1173.102786

 

Abstract

In this study, we sequenced two complete mitogenomes fromDeroceras laeveandAmbigolimax valentianus. The mitogenome ofAmbigolimax valentianusrepresented the first such data from the family Limacidae. The lengths of the mitogenomes ofDeroceras laeveandAmbigolimax valentianuswere 14,773 bp and 15,195 bp, respectively. The entire set of 37 mitochondrial genes were identified for both mitogenomes. Compared with the mitogenome ofAchatina fulica, thetrnP_trnAtRNA cluster was rearranged in bothDeroceras laeveandAmbigolimax valentianus. The secondary structures of tRNA and rRNA genes for the two species were predicted. Phylogenetic analyses based on amino acid sequences supported (1) monophyly of Stylommatophora, (2) division of Stylommatophora into the ‘achatinoid’ clade (i.e., the suborder Achatinina) and the ‘non-achatinoid’ clade (i.e., the suborder Helicina), (3) placement of the Orthurethra in the ‘non-achatinoid’ clade, and (4) monophyly of each of the superfamilies Helicoidea, Urocoptoidea, Succineoidea, Arionoidea, Pupilloidea and Limacoidea. The exemplars of Helicidae, Philomycidae and Achatinellidae displayed many more mitochondrial gene rearrangements than other species of Stylommatophora.