The larval, pupal and mitogenomic characteristics of Agrilus adelphinus Kerremans, 1895 (Coleoptera, Buprestidae) from China

By

Huang, Xuyan
Gan, Yujie
Wang, Lei
Xu, Yanying
Wei, Zhonghua
Shi, Ai-Min

Type

Article

Date of Publication

2023

Original Publication

ZooKeys

Volume

1174

Pages

15--33

Subjects

larva , Mitogenome , Pupa

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.1174.105479

 

Abstract

In this study, the larva and pupa of Agrilus adelphinus are described and illustrated. DNA barcoding (COI gene) was used to associate the larval and pupal stages with adults based on the maximum-likelihood method. In the resulting phylogenetic tree, species from the same species-group were found to be clustered on a branch with high support value. To better understand A. adelphinus, the complete mitochondrial genome of this species was also sequenced and annotated. Comparing this genome to the known mitogenomes of Agrilus species, the newly sequenced genome is shorter, with 15,732 bp. However, its whole mitogenome composition and gene orientation were consistent with that of most species of Buprestidae. In the mitogenome of A. adelphinus, the ATGATAG sequence was observed between ATP8 and ATP6, which is ATGATAA in other insect mitogenomes. Leu2, Phe, Ile, Gly, and Ser2 were the five most frequently encoded amino acids. The results further prove that DNA barcoding can remove the limitation of traditional taxonomy which cannot identify to species all developmental stages. This study also provides valuable molecular and morphological data for species identification and phylogenetic analyses of the genus Agrilus.