New species of the genus Trichosetodes Ulmer, 1915 (Trichoptera, Leptoceridae) from Ratanakiri province, Cambodia, based on morphological and molecular data

By

Laudee, Pongsak
Malicky, Hans
Kong, Chamroeun
Takenaka, Masaki
Tojo, Koji

Type

Article

Date of Publication

2023

Original Publication

ZooKeys

Volume

1182

Pages

153--164

Subjects

Aquatic insects , Biodiversity , caddisfly , Mekong River basin , Morphology , ribosomal mRNA , taxonomy

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.1182.105716

 

Abstract

Three new species of Trichosetodes, namely T. carmelaesp. nov., T. katiengensissp. nov. and T. ratanakiriensissp. nov. are described and illustrated by male specimens. The male genitalia of T. carmelaesp. nov. can be distinguished from the other 16 species of the genus found in Southeast Asia by the shape of the phallicata. The phallicata of T. carmelaesp. nov. bears a tuft of long hairs in the middle of the dorsal edge. Trichosetodes katiengensissp. nov. can be distinguished from the other species in Southeast Asia by the shape of the phallicata which is divided into dorsal and ventral branches in lateral view, and T. ratanakiriensissp. nov. by the characters of the left inferior appendage and the shape of segment IX. The posterior end of the left inferior appendage of T. ratanakiriensissp. nov. is not forked and the ventral and lateral views of the posteroventral lobes of segment IX are rounded. Illustrations of male genitalia of Trichosetodes kampongspeuensis Malicky & Kong, 2020 are provided for comparison. The molecular diversity of new Trichosetodes species was analyzed using the mitochondrial large subunit ribosomal rRNA gene region (16S rRNA). In terms of their genetic divergence, T. ratanakiriensissp. nov. and T. kampongspeuensis exhibited remarkable proximity, with only a 1.4% distance. On the contrary, T. carmelaesp. nov. displayed genetic disparity exceeding 6.3% when compared to both T. ratanakiriensissp. nov. and T. kampongspeuensis.