Prospects and possibilities of using Raman spectroscopy for the identification of Pseudomonas aeruginosa from turtle Emys orbicularis (Linnaeus, 1758) skin

By

Petjukevics, Aleksandrs
Umbrasko, Inta
Skute, Natalja

Type

Article

Date of Publication

2023

Original Publication

BioRisk : biodiversity & ecosystem risk assessment

Volume

21

Pages

19--28

Subjects

Bacteria , identification of microorganisms , Pseudomonas aeruginosa , Raman spectroscopy , Reptiles , turtle

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft

Identifiers

DOI: https://doi.org/10.3897/biorisk.21.111983

 

Abstract

This study describes an express method for identifying microorganisms: Pseudomonas aeruginosa by standard Raman spectroscopy, without surface-enhanced Raman spectroscopy (SERS). The short-wavelength 514 nm Ar-Ion laser was used for P. aeruginosa spectral identification in the Raman shift range from 3200 cm−1 to 200 cm−1. The research results showed a high analytical and diagnostic sensitivity of the technology to the express identification of P. aeruginosa and can be used as one of the reliable methods. The proven technology is promising for further research of other microorganisms due to several significant advantages of the method. It does not require long-term cultivation of bacteria and special sample preparation, additional expensive reagents or consumables.