Karyotype analysis of Quasipaa spinosa David, 1875 (Anura, Dicroglossidae) with conventional cytogenetic techniques

By

Zhang, Liaoruilin
Xiang, Jianguo
Li, Juan
Zhou, Jie
Hou, Jinliang
Huang, Yanfei
Li, Hong

Type

Article

Date of Publication

2024

Original Publication

Comparative cytogenetics : international journal of plant & animal cytogenetics, karyosystematics and molecular systematics

Volume

18

Pages

97--103

Subjects

BrdU-banding , C-banding , karyotype , Quasipaa spinosa

Contributed by

Pensoft Publishers

Language

English

Published by

St. Petersburg, Russia [Published] with technical support of Zoological Institute RAS [2007]-

Identifiers

DOI: https://doi.org/10.3897/compcytogen.18.116806

 

Abstract

The current study analyzed the chromosomal karyotype of Quasipaa spinosa David, 1875 from Hunan Province, China. The karyotype, C-banding, BrdU-banding pattern were characterized using direct preparation of bone-marrow cells and hemocyte cultures. The findings indicated that Q. spinosa was a diploid species (2n = 26) that lacked heteromorphic chromosomes and secondary constrictions. C-banding analysis revealed an abundance of positive signals in the centromere regions, while the BrdU-banding pattern showed three phases in both male and female, occurring consistently and in chronological sequence during S-phase. Notably, there was no asynchronous replication in the late phase. This study enhanced our understanding of the karyotypic structure of Q. spinosa by conventional cytogenetic techniques, thus providing essential scientific insights into the cytogenetics of Q. spinosa.