In the face of ongoing anthropogenic pressure and biodiversity loss, there is a need to protect nature more effectively. Therefore, we propose a comprehensive and consecutive approach utilising landscape ecological principles and methods for effective landscape protection and spatial nature conservation. Methods applicable in various conditions are exemplified through case studies from the Šumava National Park, the largest NP in Czechia. Using a set of spatial environmental, landscape ecological and geographical data we can:<br> Characterise the area of interest from the physical-geographical, socioeconomic, and management point of view to create the concept´s framework and review important background information for analysis of the area. Therefore, the key factors for landscape protection and biodiversity conservation are defined.<br> Analyse trends and processes of landscape dynamics in terms of land cover, landscape structure and habitat fragmentation and connectivity, which helps us to set main objectives of landscape protection and nature conservation.<br> Use data about environment conditions and key species and habitat occurrence to model habitat suitability, identify their suitable areas, and thus improve their protection. As a result, areas of high conservation value are distinguished.<br> Synthetize outputs of the above-mentioned steps and prioritise the target goals of landscape protection and biodiversity conservation in the area of interest. This leads to the effective zonation, which is a necessary condition for the application of appropriate management measures.
