The first record of Chironomus nuditarsis Keyl, 1961 from Sevan Lake (Armenia) confirmed by morphology, karyotype and COI gene sequence

By

Bolshakov, Viktor
Prokin, Alexander
Ivanova, E S
Movergoz, Ekaterina

Type

Article

Date of Publication

2024

Original Publication

Comparative cytogenetics : international journal of plant & animal cytogenetics, karyosystematics and molecular systematics

Volume

18

Pages

123--141

Subjects

Barcode , Chironomus nuditarsis , COI , Diptera , karyotype , Lake Sevan

Contributed by

Pensoft Publishers

Language

English

Published by

St. Petersburg, Russia [Published] with technical support of Zoological Institute RAS [2007]-

Identifiers

DOI: https://doi.org/10.3897/compcytogen.18.126130

 

Abstract

Chironomus nuditarsis Keyl, 1961 is recorded from Sevan Lake for the first time. This species is widespread in Europe, the Caucasus, and Siberia. For species identification, we used a comprehensive approach that included morphological, cytogenetic and molecular genetic analyses. Morphological analysis showed a high similarity with the description. Nine chromosome banding sequences ndtA1, ndtA2, ndtB2, ndtC1, ndtD1, ndtE1, ndtF1, ndtG1, and ndtG2 were found. The banding sequences ndtA1, ndtA2, ndtG1, and ndtG2 are species-specific for C. nuditarsis and allow us to accurately distinguish it from the sibling species Ch. curabilis Belyanina, Sigareva et Loginova, 1990. Molecular-genetic analysis of the COI gene sequences has shown low genetic distances of 0.38–0.95% in the sibling species Ch. nuditarsis and Ch. curabilis complex and the insufficiency of using a single COI as a molecular marker for their separation.