Saccharomyces cerevisiae yeast cells as a test system for assessing Zeocin toxicity

By

Marinovska, Polya
Todorova, Teodora Ivanova
Tomova, Anna
Pisareva, Emiliya
Boyadzhiev, Krassimir
Dimitrov, Martin
Parvanova, Petya
Dimitrova, Maria
Chankova, Stephka
Petrova, Ventsislava

Type

Article

Date of Publication

2022-4-21

Original Publication

BioRisk : biodiversity & ecosystem risk assessment

Volume

17

Pages

105--116

Subjects

quiescence , Stress response , Yeast , Zeocin

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft

Identifiers

DOI: https://doi.org/10.3897/biorisk.17.77227

 

Abstract

Having unique genetic machinery and a high degree of conservation with higher eukaryotes, the yeast Saccharomyces cerevisiae is recognised as a smart experimental system for studying the modes of chemical toxicity. The present study was undertaken to elucidate the changes in the intracellular redox homeostasis and key macromolecule structure following exposure to Zeocin. Cell populations of logarithmic, quiescent (Q) and non-quiescent (NQ) cells of Saccharomyces cerevisiae BY4741 were used as a model to examine the cytotoxic effect of this radiomimetic. The levels of endogenous ROS, oxidized lipids, carbonylated proteins, and glutathione were analysed after treatment with Zeocin (IC50). An increase in ROS production and respectively increased oxidative stress was detected in all three types of cell populations, with the highest degree being observed in proliferating S. cerevisiae BY4741 cells. The stress response of both proliferating and stationary phase (Q and NQ) cells to Zeocin included an overexpression of glutathione. The quiescent cells also showed very low DNA susceptibility to high Zeocin concentration (100–300 µg/ml), presented as no induced double-strand breaks (DSBs) in the macromolecule. Based on our research it could be concluded that the cellular physiological state is a critical factor determining the resistance to environmental stress with Q cells being the most robust.