In vitro reconstitution of complexes of stress HliA protein with pigments

By

Sharapova, Lubov
Yurina, Nadezhda P.

Type

Article

Date of Publication

2022-4-21

Original Publication

BioRisk : biodiversity & ecosystem risk assessment

Volume

17

Pages

139--146

Subjects

Carotenoid , Chlorophyll , Cyanobacteria , high light inducible proteins , high light stress , Synechocystis

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft

Identifiers

DOI: https://doi.org/10.3897/biorisk.17.77306

 

Abstract

Proteins similar to Hli (high light inducible) proteins of cyanobacteria are present in all photosynthetic eukaryotes and are necessary for survival in various stressful conditions, although their exact function is not fully understood. In current study, the recombinant stress-induced protein HliA of cyanobacterium Synechocystis was isolated and characterised for the first time. The synthetic gene of HliA protein was created and cloned into plasmid for expression of recombinant protein with Hisx6-tag at the C-terminus in bacteria. Recombinant HliA protein of Synechocystis was isolated by metal-affinity chromatography. The HliA protein was reconstituted with chlorophyll a and carotenoids. Using circular dichroism spectroscopy, it was shown that chlorophyll a and carotenoids interact in vitro with the HliA protein. The binding of pigments to the HliA protein favours the protective function of this protein. Apparently, Hli proteins are involved in the coordinated delivery of pigments for the biogenesis of photosynthetic complexes, thereby reducing the risk of accumulation of phototoxic free chlorophyll molecules. Current results are important for understanding the processes of photoprotection in either cyanobacteria or algae and higher plants.