The InBIO barcoding initiative database: DNA barcodes of Iberian Trichoptera, documenting biodiversity for freshwater biomonitoring in a Mediterranean hotspot

By

Paupério, Joana
Martin, Luis
Martínez, Jesús
Gonzalez, Marcos
Martins, Filipa Ms
Veríssimo, Joana
Puppo, Pamela.
Pinto, Joana
Chaves, Cátia
Pinho, Catarina J.
Grosso-Silva, José Manuel
Quaglietta, Lorenzo
Silva, Teresa Luísa
Sousa, Pedro
Alves, Paulo
Fonseca, Nuno
Beja, Pedro
Ferreira, Sónia

Type

Article

Date of Publication

2023-01-19

Original Publication

Biodiversity data journal

Volume

11

Pages

1--36

Subjects

continental Portugal , continental Spain , Cytochrome c oxidase subunit I (COI) , DNA barcode , occurrence records , species distributions , Trichoptera

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia Pensoft Publishers [2013]-

Identifiers

DOI: https://doi.org/10.3897/BDJ.11.e97484
eLocator: e97484

 

Abstract

The Trichoptera are an important component of freshwater ecosystems. In the Iberian Peninsula, 380 taxa of caddisflies are known, with nearly 1/3 of the total species being endemic in the region. A reference collection of morphologically identified Trichoptera specimens, representing 142 Iberian taxa, was constructed. The InBIO Barcoding Initiative (IBI) Trichoptera 01 dataset contains records of 438 sequenced specimens. The species of this dataset correspond to about 37% of Iberian Trichoptera species diversity. Specimens were collected between 1975 and 2018 and are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources, Portugal) or in the collection Marcos A. González at the University of Santiago de Compostela (Spain).Twenty-nine species, from nine different families, were new additions to the Barcode of Life Data System (BOLD). A success identification rate of over 80% was achieved when comparing morphological identifications and DNA barcodes for the species analysed. This encouraging step advances incorporation of informed Environmental DNA tools in biomonitoring schemes, given the shortcomings of morphological identifications of larvae and adult Caddisflies in such studies. DNA barcoding was not successful in identifying species in six Trichoptera genera:Hydropsyche(Hydropsychidae),Athripsodes(Leptoceridae),Wormaldia(Philopotamidae),Polycentropus(Polycentropodidae)Rhyacophila(Rhyacophilidae) andSericostoma(Sericostomatidae). The high levels of intraspecific genetic variability found, combined with a lack of a barcode gap and a challenging morphological identification, rendered these species as needing additional studies to resolve their taxonomy.