Diversity of culturable thermophilic bacteria from Tata Pani hotspring of Kotli Azad Jammu and Kashmir

By

Ishaq, Kazima
Shah, Asad
Fariq, Anila
Rasheed, Sajida
Jannat, Sammyia

Type

Article

Date of Publication

2023-03-14

Original Publication

Biodiversity data journal

Volume

11

Pages

1--18

Subjects

16S rDNA , archaea , Bacteria , diversity , Hot water springs , thermophiles

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia Pensoft Publishers [2013]-

Identifiers

DOI: https://doi.org/10.3897/BDJ.11.e99224
eLocator: e99224

 

Abstract

Hot water springs are unique areas populated by mesophiles, thermotolerant and hyperthermophiles. They are the source of diversity of thermophiles, mainly belonging to archaea and bacteria domains. The diversity of thermophiles gives an outline of the huge biological potential that can be exploited for industrial applications.To this end, this study was aimed to isolate and characterise the unexplored thermophilic microorganisms from hot water spring in Tatapani, Tehsil & District Kotli AJK, Pakistan. Around 10 bacterial isolates were identified using morphological, biochemical, physiological and molecular attributes. Sequencing of the 16S rDNA gene of the isolates followed by BLAST search revealed that the strain MBT008 has 100% similarity with Anoxybacillus kamchatkensis. MBT012 showed 99.57% similarity with A. mongoliensis, MBT014 was affiliated with A. tengchongensis with 99.43% similarity, MBT009 showed 99.83% homology with A. gonensis and MBT018, 98.70% similarity with A. karvacharensis. The presence of all this microbial diversity in one common source is of immense importance related to envioronmental and industrial aspects in general and extraction of thermostable enzymes from these thermophiles specifically opens new horizons in the field of industrial biotechnology. These thermophiles are revealing new capabilities and are being manipulated by biotechnologists in utilizing them in different unique ways.