Morphological and molecular characterization of Halamphora vantushpaensis (Bacillariophyceae, Amphipleuraceae), a new diatom species widely dispersed on the shores of the soda Lake Van (Türkiye)

By

Yilmaz, Elif
Gastineau, Romain
Solak, Cuneyt Nadir
Górecka, Ewa
Trobajo, Rosa
Turmel, Monique
Lemieux, Claude
Otis, Christian
Witkowski, Andrzej
Mann, David G.

Type

Article

Date of Publication

2024

Original Publication

PhytoKeys

Volume

249

Pages

95--114

Subjects

Alkaline lake , group II introns , inverted repeat , mitochondrial genome , multigene phylogeny , nuclear rRNA genes , Plastid genome

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers [2010]-

Identifiers

DOI: https://doi.org/10.3897/phytokeys.249.133205

 

Abstract

In this study, we describe Halamphora vantushpaensissp. nov., a newly identified diatom species found in the highly alkaline Lake Van in Eastern Turkey (Türkiye). This new species is characterized morphologically by light and scanning electron microscopy, performed on both wild and cultivated samples. Two monoclonal cultures were submitted to a genome-skimming approach, giving access to the complete sequence of their nuclear rRNA cluster of genes, mitochondrial and plastid genomes. Both strains were highly similar from the genomic point of view, with few mutations noted, although in organellar genomes some of them concerned protein coding genes and were non-silent. Also, the group II intron in the mitochondrial cox1 gene was found to display a relatively high number of polymorphisms. The plastome also distinguishes itself from other Halamphora spp. by the extension of its inverted repeat at the expense of the two single copy regions of the genome. Maximum likelihood molecular phylogeny inferred from a concatenated three genes dataset (18S, psbC and rbcL) positions this species within the K clade, which is known to contain hypersaline to freshwater species.