Complete mitochondrial genome of Lepidocephalichthys berdmorei and its phylogenetic status within the family Cobitidae (Cypriniformes)

By

Zhou, Min
Wang, Cheng
Xu, Ziyue
Zhong hua jiao yu wen hua ji jin dong shi hui bian yi wei yuan hui 中华教育文化基金董事会编译委员会
He, Yang
Wang, Ying (Botanist)

Type

Article

Date of Publication

2024

Original Publication

ZooKeys

Volume

1221

Pages

51--69

Subjects

Gene arrangement pattern , Lepidocephalichthys berdmorei , mitochondrial genome , phylogenetic analysis

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.1221.129136

 

Abstract

In this study, the complete mitochondrial genome of Lepidocephalichthys berdmorei was first determined by the primer walking sequence method. The complete mitochondrial genome was 16,574 bp in length, including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, and a control region (D-loop). The gene arrangement pattern was identical to that of other teleosts. The overall base composition was 29.9% A, 28.5% T, 25.5% C, and 16.1% G, with an A+T bias of 58.4%. Furthermore, phylogenetic analyses were conducted based on 13 PCGs from the mitochondrial genomes of 18 cobitid species using with three different methods (Neighbor-joining, Maximum likelihood, and Bayesian inference). All methods consistently showed that the four species of the genus Lepidocephalichthys form a monophyletic group. This study would provide effective molecular information for the Lepidocephalichthys species as well as novel genetic marker for the study of species identification.