PASSIFOR: A reference library of DNA barcodes for French saproxylic beetles (Insecta, Coleoptera)

By

Rougerie, Rodolphe, 1974-
Lopez-Vaamonde, Carlos
Barnouin, Thomas
Delnatte, Julien
Moulin, Nicolas
Noblecourt, Thierry
Nusillard, Benoît
Parmain, Guillem
Soldati, Fabien
Bouget, Christophe

Type

Article

Date of Publication

2015-03-04

Original Publication

Biodiversity data journal

Volume

3

Pages

1--19

Subjects

COI , Coleoptera , Cryptic Diversity , DNA barcoding , ecological indicators. , Forest insects , molecular identification

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia Pensoft Publishers [2013]-

Identifiers

DOI: https://doi.org/10.3897/BDJ.3.e4078
eLocator: e4078

 

Abstract

Saproxylic beetles – associated with dead wood or with other insects, fungi and microorganisms that decompose it – play a major role in forest nutrient cycling. They are important ecosystem service providers and are used as key bio-indicators of old-growth forests. In France alone, where the present study took place, there are about 2500 species distributed within 71 families. This high diversity represents a major challenge for specimen sorting and identification.<br>The PASSIFOR project aims at developing a DNA metabarcoding approach to facilitate and enhance the monitoring of saproxylic beetles as indicators in ecological studies. As a first step toward that goal we assembled a library of DNA barcodes using the standard genetic marker for animals, i.e. a portion of the COI mitochondrial gene. In the present contribution, we release a library including 656 records representing 410 species in 40 different families. Species were identified by expert taxonomists, and each record is linked to a voucher specimen to enable future morphological examination. We also highlight and briefly discuss cases of low interspecific divergences, as well as cases of high intraspecific divergences that might represent cases of overlooked or cryptic diversity.