A streamlined collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, exemplified by the Indonesian Biodiversity Discovery and Information System (IndoBioSys)

By

Schmidt, Olga
Hausmann, Axel
Cancian de Araujo, Bruno
Sutrisno, Hari
Peggie, Djunijanti
Schmidt, Stefan

Type

Article

Date of Publication

2017-10-31

Original Publication

Biodiversity data journal

Volume

5

Pages

1--14

Subjects

collecting methods , DNA barcoding , light trap , Monitoring , Moths , rapid biodiversity assessment , sampling protocol

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia Pensoft Publishers [2013]-

Identifiers

DOI: https://doi.org/10.3897/BDJ.5.e20006
eLocator: e20006

 

Abstract

Here we present a general collecting and preparation protocol for DNA barcoding of Lepidoptera as part of large-scale rapid biodiversity assessment projects, and a comparison with alternative preserving and vouchering methods. About 98% of the sequenced specimens processed using the present collecting and preparation protocol yielded sequences with more than 500 base pairs. The study is based on the first outcomes of the Indonesian Biodiversity Discovery and Information System (IndoBioSys). IndoBioSys is a German-Indonesian research project that is conducted by the Museum für Naturkunde in Berlin and the Zoologische Staatssammlung München, in close cooperation with the Research Center for Biology – Indonesian Institute of Sciences (RCB-LIPI, Bogor).