Microsatellite repeat mapping shows inner chromosomal diversification in highly conserved karyotypes of Asian cyprinid fishes

By

Buasriyot, Phichaya
Supiwong, Weerayuth
Muanglen, Nawarat
Donbundit, Nattasuda
Ditcharoen, Sukhonthip
Chumpol, Phonluang
Saenjundaeng, Pasakorn
Tongnunui, Sampan
Arunsang, Sathit
Thongnetr, Weera
Juntharat, Sitthisak
Seetapan, Kriengkrai
Liehr, Thomas 1965-
Cioffi, Marcelo de Bello
Rab, Petr
Tanomtong, Alongklod

Type

Article

Date of Publication

2025

Original Publication

Comparative cytogenetics : international journal of plant & animal cytogenetics, karyosystematics and molecular systematics

Volume

19

Pages

29--50

Subjects

chromosomal rearrangements , Comparative cytogenetics , Family Cyprinidae , fluorescence in situ hybridization (FISH) , microsatellites

Contributed by

Pensoft Publishers

Language

English

Published by

St. Petersburg, Russia [Published] with technical support of Zoological Institute RAS [2007]-

Identifiers

DOI: https://doi.org/10.3897/compcytogen.19.141557

 

Abstract

The barbels of the subfamilies ´Poropuntinae´ and Smiliogastrinae within the family Cyprinidae play a significant role as a food source for fish in artisanal fisheries and are highly valued as ornamental fish in Thailand. In this study, we employed both conventional and molecular cytogenetics to analyze the karyotype of 15 fish species from two cyprinid lineages. All analyzed species had a diploid chromosome number of 2n = 50. Despite sharing the same 2n, our analyses revealed species-specific distribution patterns of the mapped microsatellite motifs [(CA)₁₅, (TA)₁₅, (CAC)₁₀, and (CGG)₁₀]. They were predominantly found at telomeric sites of all-to-few chromosomes. Additionally, some species exhibited a widespread distribution of the mapped microsatellites across the chromosomes while others showed no signal. These variations reflect the evolutionary divergence and chromosomal diversity within the cyprinids. Thus, our findings support the 2n stability in cyprinoid lineages while emphasizing the intrachromosomal evolutionary diversity accompanied by species-specific microsatellite distribution.