Comparison of different cytogenetic methods and tissue suitability for the study of chromosomes in Cimex lectularius (Heteroptera, Cimicidae)

By

Sadílek, David
Angus, Robert B.
Šťáhlavský, František
Vilímová, Jitka

Type

Article

Date of Publication

2016-12-14

Original Publication

Comparative cytogenetics : international journal of plant & animal cytogenetics, karyosystematics and molecular systematics

Volume

10

Series / Issue

Issue: 4

Pages

731--752

Subjects

holokinetic chromosomes , karyogram , Karyogram , midgut , spreading method , squashing method , Testes

Contributed by

Pensoft Publishers

Language

English

Published by

St. Petersburg, Russia [Published] with technical support of Zoological Institute RAS [2007]-

Identifiers

DOI: https://doi.org/10.3897/CompCytogen.v10i4.10681

 

Abstract

In the article we summarize the most common recent cytogenetic methods used in analysis of karyotypes in Heteroptera. We seek to show the pros and cons of the spreading method compared with the traditional squashing method. We discuss the suitability of gonad, midgut and embryo tissue inCimexlectulariusLinnaeus, 1758 chromosome research and production of figures of whole mitosis and meiosis, using the spreading method.The hotplate spreading technique has many advantages in comparison with the squashing technique. Chromosomal slides prepared from the testes tissue gave the best results, tissues of eggs and midgut epithelium are not suitable. Metaphase II is the only division phase in which sex chromosomes can be clearly distinguished. Chromosome number determination is easy during metaphase I and metaphase II. Spreading of gonad tissue is a suitable method for the cytogenetic analysis of holokinetic chromosomes ofCimexlectularius.