Metadata standards and practical guidelines for specimen and DNA curation when building barcode reference libraries for aquatic life

By

Rimet, Frederic
Aylagas, Eva
Borja, Ángel.
Bouchez, Agnès
Canino, Alexis
Chauvin, Christian
Chonova, Teofana
Čiampor, Fedor
Costa, Filipe O.
Ferrari, Benoit J. D.
Gastineau, Romain
Goulon, Chloé
Gugger, Muriel
Holzmann, Maria
Jahn, Regine
Kahlert, Maria
Kusber, Wolf-Henning
Laplace-Treyture, Christophe
Leese, Florian 1978-
Leliaert, Frederik
Mann, David G.
Marchand, Frédéric
Méléder, Vona
Pawlowski, Jan
Rasconi, Serena
Rivera, Sinziana
Rougerie, Rodolphe, 1974-
Schweizer, Magali
Trobajo, Rosa
Vasselon, Valentin
Vivien, Régis
Weigand, Alexander M., 1984-
Witkowski, Andrzej
Zimmermann, Jonas
Ekrem, Torbjørn 1973-

Type

Article

Date of Publication

2021-2-26

Original Publication

Metabarcoding and metagenomics

Volume

5

Pages

1--17

Subjects

Aquatic organisms , Barcode , DNA , metadata , Quality , reference library , traceability

Contributed by

Pensoft Publishers

Language

English

Published by

Bulgaria Pensoft Publishers 2017-

Identifiers

DOI: https://doi.org/10.3897/mbmg.5.58056

 

Abstract

DNA barcoding and metabarcoding is increasingly used to effectively and precisely assess and monitor biodiversity in aquatic ecosystems. As these methods rely on data availability and quality of barcode reference libraries, it is important to develop and follow best practices to ensure optimal quality and traceability of the metadata associated with the reference barcodes used for identification. Sufficient metadata, as well as vouchers, corresponding to each reference barcode must be available to ensure reliable barcode library curation and, thereby, provide trustworthy baselines for downstream molecular species identification. This document (1) specifies the data and metadata required to ensure the relevance, the accessibility and traceability of DNA barcodes and (2) specifies the recommendations for DNA harvesting and for the storage of both voucher specimens/samples and barcode data.