Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity

By

Rajendra, P
Sujatha, H
Sashidhar, R
Subramanyam, C
Devendranath, D
Aradhya, R

Type

Article

Date of Publication

2012-8-6

Original Publication

Nature conservation

Volume

2

Pages

1--11

Subjects

apoptosis , caspase 9 , DNA damage , ELF-EMF , primary lymphocytes

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers 2012-

Identifiers

DOI: https://doi.org/10.7750/BioDiscovery.2012.2.2

 

Abstract

The cell viability and DNA damage in unstimulated sheep primary lymphocytes subjected to different extremely low electromagnetic field intensities (5, 50 and 100 µT; 50 Hz) were studied with special emphasis on apoptosis. Sheep primary lymphocytes cultured in RPMI, supplemented with 10% FBS in the absence of mitogens, were exposed till 16 h. The cell viability assessment by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay showed a dose dependent enhancement of viability at 16 h. Further, quantitative DNA laddering and flow cytometric analysis showed a significant decrease in apoptosis of the cells subjected to 100 (p