Chromosome rearrangements in sublines of human embryonic stem cell lines hESM01 and hESM03

By

Karamysheva, Tatyana
Prokhorovich, Мaria
Lagarkova, Maria
Kiselev, Sergey
Liehr, Thomas 1965-
Rubtsov, Nikolay

Type

Article

Date of Publication

2013-3-

Original Publication

Biodiscovery

Volume

7

Series / Issue

Issue: 7

Pages

1--5

Subjects

chromosome rearrangements , fluorescent in situ hybridization (FISH) , human embryonic stem cells (hESCs) , Microdissection , microdissection , microdissection , multicolor banding , reverse FISH

Contributed by

Pensoft Publishers

Language

English

Published by

Newport on Tay Scottish Science Press

Identifiers

DOI: https://doi.org/10.7750/BioDiscovery.2013.7.1

 

Abstract

Due to possible proliferative effects of karyotypic reorganization of human embryonic stem cell (hESC) lines detailed genetic analysis are indicated prior to any application of hESCs. Molecular cytogenetic analysis of two different hESC sublines was performed and revealed aberrant chromosomes in both of them, i.e. in hESM01r18 (46,ХХ,-18,+mar) and hESM0309 (46,ХХ,del(4),dup(9)). This study shows that microdissection and multicolor fluorescence <em>in situ</em> hybridization (mFISH) can be used to detect the chromosomal changes precisely of the derivative chromosomes that are difficult to identify by conventional G-banded chromosome analysis. In the present study chromosome microdissection and reverse FISH were applied using multicolor fluorescence <em>in situ</em> hybridization (mFISH) for detailed characterization of the derivative chromosomes. The karyotypes of hESC lines were described as: 46,ХХ,r(18)(::p11.31→q21.2::q21.2→p11.31::) and 46,XX,del(4)(q25q31.1),dup(9)(q12q33), respectively. The potential role of the chromosomal regions involved in rearrangements for cell proliferation is discussed.