Blood-Based Gene Expression in children with Autism spectrum disorder

By

Doflein, Franz, 1873-1924
Ivanov, Hristo
Stoyanova, Vili
Doflein, Franz, 1873-1924
Popov, Nikolay
Doflein, Franz, 1873-1924
Bosheva, M
Doflein, Franz, 1873-1924
Vachev, Tihomir
Doflein, Franz, 1873-1924

Type

Article

Date of Publication

2015-9-30

Original Publication

Biodiscovery

Volume

17

Series / Issue

Issue: 17

Pages

1--7

Subjects

ASD , gene expression , gene pathway analysis , transcriptome sequencing

Contributed by

Pensoft Publishers

Language

English

Published by

Newport on Tay Scottish Science Press

Identifiers

DOI: https://doi.org/10.7750/BioDiscovery.2015.17.2

 

Abstract

Comparative gene expression profiling analysis is useful in discovering differentially expressed genes associated with various diseases, including mental disorders. Autism spectrum disorder (ASD) is a severe neuropsychiatric disorder which has complex pathobiology with profound influences of genetic factors in its development. Although numerous autism susceptible genes were identified, the etiology of autism is not fully explained. The study aimed to examine gene expression profiling in peripheral blood from 60 individuals divided into two groups: children with ASD and age- and gender-matched healthy subjects (ASD control). A genome-wide sequencing of copy DNA molecules was conducted to obtain information for quantitative expression of all genes subject to this study and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway-based analysis was used to further understand genes’ biological functions. Based on the conducted expression analysis 23 differentially expressed genes and 21 KEGG signaling pathways with statistical significant change were identified. Blood-based comparative gene expression profiling analysis is useful in discovering genetic markers associated with ASD. Our data will provide a valuable resource for discovery purposes and for comparison to other gene expression-based, genome-wide studies and other functional data.