In vitro assessment of the cytotoxic effects of novel RGD analogues

By

Balacheva, Anelia
Iliev, Ivan
Detcheva, Roumyana
Dzimbova, Tatyana
Pajpanova, Tamara
Golovinsky, Evgeny

Type

Article

Date of Publication

2012-10-

Original Publication

MycoKeys

Volume

4

Pages

1--6

Subjects

canavanine , cytotoxic activity , RGD

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers 2011-

Identifiers

DOI: https://doi.org/10.7750/BioDiscovery.2012.4.1

 

Abstract

The RGD sequence is present in many extracellular matrix proteins and intracellular proteins, including caspases. Synthetic RGD peptides may affect adhesion, migration and tumour metastasis, or directly induce apoptosis. Several RGD peptides were synthesized, and their anti-adhesive and cytotoxic properties were analyzed in vitro. Here we present the cytotoxic activities of RGD, R(NO2)GD, CavGD and RGD-OMe on non-tumour 3T3 cells and tumour cell lines HepG2 and MCF-7. The cell growth inhibitory effects of RGD-OMe are significantly higher than those of RGD on the cell lines used. Evidently the modification in the carboxylic group of RGD with simple esterification increases the cell growth inhibitory effects of the parent compound.