Characterization of the genetic diversity of a population of Odocoileus virginianus veraecrucis in captivity using microsatellite markers

By

Castillo-Rodríguez, Ruth Guadalupe
Lagunes, Ricardo
Cruz-Romero, Anabel
Núñez-Pastrana, Rosalía
Rojas-Avelizapa, Luz Irene
Régulo, Carlos Llarena-Hernández
Dávila, José Antonio

Type

Article

Date of Publication

2020-2-6

Original Publication

Neotropical Biology and Conservation

Volume

15

Series / Issue

Issue: 1

Pages

29--41

Subjects

allelic diversity , Conservation , effective population size , Veracruz white-tail deer

Contributed by

Pensoft Publishers

Language

English

Published by

Brazil Universidade do Vale do Rio dos Sinos

Identifiers

DOI: https://doi.org/10.3897/neotropical.15.e47262

 

Abstract

The genetic diversity and effective population size (Ne) of a population ofOdocoileus virginianus veraecrucisin captivity were characterized in the Wildlife Management Unit “El Pochote”, located in Ixtaczoquitlán, Veracruz, Mexico. Blood tissue was collected from 20 individuals of the reproductive nucleus, its genomic DNA was extracted, and genetic diversity was characterized by six microsatellites amplified by PCR and visualized in polyacrylamide gels. With four polymorphic microsatellites, 66.7% of the population’s genetic variation was explained, which was characterized by an allelic diversity that fluctuated between 9 and 28 alleles (18 average alleles), suggesting a mean allelic diversity (Shannon index = 2.6 ± 0.25), but only 12 ± 2.9 effective alleles would be fixed in the next generation. The heterozygosity observed (Ho= 0.81) exceeded that expected (He= 0.79) and these were significantly different (P> 0.05), as a result of a low genetic structure in the population (fixation indexF= -0.112 ± 0.03), due to the genetic heterogeneity that each sample contributed, since the specimens came from different geographical regions. The Ne was 625 individuals and a 1:25 male:female ratio, with which 100% of the genetic diversity observed can be maintained for 100 years. The information obtained in the study can help in the design of a reproductive management program to maintain the present genetic diversity, without risk of losses due to genetic drift and inbreeding.