Perennial wildflower meadows contribute to the enhancement of the quality of urban green spaces, of-fering benefits for both people and wildlife. Significant impact is attributed to management in fostering species-diverse plant communities. However, there are not enough studies exploring the influence of early maintenance regimes on plant diversity. A three-year experiment was conducted to investigate the impact of maintenance regimes on newly established perennial wildflower meadows and the influence of sowing mix on species richness. Three types of perennial wildflower seed mixes were sown on arable land and managed differently during the initial year. Results indicate statistical significance for each main effect, including maintenance type and sowing mix, with no significant interaction between these factors. Mowing during the early growth stage is shown to increase species richness, while no significant difference is observed between weeded and unmanaged plots. The dry meadow sowing mix demonstrates the highest diversity index (Shannon-Weaver, 1963). Maintenance regimes exhibit a significant influence on species richness independently of the sowing mix used. Furthermore, mowing during the early growth stage is found to enhance species diversity in the long term. These findings provide beneficial insights for the more efficient management and establishment of species-rich wildflower perennial meadows in both urban and suburban environments, particularly in areas with a significant soil weed seed bank.
