Traccatichthys punctulatus is here described from the Liu-Jiang flowing into the Xi-Jiang of the Pearl River basin at Jinxiu County, Guangxi Zhuang Autonomous Region, southern China. It is distinguished from all congeners by having a unique body coloration of a series of brown spots along the lateral line and dorsal-fin. Other diagnostic characters, not unique to this new species, are caudal-fin and lateral body colorations, and absence of pectoral-fin tuberculation. Its validity is further confirmed by its monophyly recovered in a molecular phylogenetic analysis based on mitochondrial COI and Cyt b gene sequences and species-level divergence between the new species and its closest relatives.