Comparative analysis of mitogenomes in six Scolopendra species (Chilopoda, Scolopendromorpha): insights into rare genetic rearrangements and phylogeny

By

Wan, Mengcheng
Yang, Yiwen
Wang, Jiachen
Zhang, Gaoji
Liu, Hongyi
Gao, Cuiqing

Type

Article

Date of Publication

2025-8-11

Original Publication

ZooKeys

Volume

1248

Pages

341--358

Subjects

Centipede , Centipede , gene duplication , gene rearrangement , mitochondrial genome , mitochondrial genome , mitochondrial genome , mitochondrial genome , mitochondrial genome , mitogenomic structure , Phylogeny , Scolopendridae , Scolopendrinae

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.1248.159578

 

Abstract

Six complete mitochondrial genomes from species within Scolopendra (Scolopendromorpha) (S. alcyona, S. calcarata, S. cataracta, S. lufengia, S. mazbii, and S. multidens) were analyzed. The mitochondrial genomes ranged in size from 14,422 bp to 15,458 bp, with A+T content varying between 67.00% and 74.53%. All PCGs and rRNA were successfully identified, though tRNAs exhibited widespread loss. Additionally, supplementary control region sequences were identified in three species. Beyond sequence data, the arrangement of mitochondrial genes can provide additional phylogenetically relevant information. The mitogenome arrangements in S. alcyona, S. cataracta, S. lufengia, and S. mazbii are consistent with the inferred ancestral arrangement in Myriapoda, but Scolopendra multidens had a duplication of tRNA and S. calcarata underwent a significant gene rearrangement. The Tandem Duplication/Random Loss model and tRNA mispriming mode were determined as most likely explanations for the observed gene rearrangements. Phylogenetic trees were reconstructed using the six newly sequenced mitogenomes and published mitochondrial data from other Chilopoda species. The results revealed two major monophyletic clades within Scolopendra: one comprising S. alcyona, S. dehaani, S. cataracta, S. lufengia, S. morsitans, S. multidens, and S. mutilans, and the other uniting S. mazbii and S. calcarata. Notably, S. subspinipes unexpectedly clustered with Scolopocryptops sp., challenging the monophyly of Scolopendra and highlighting the need for revised taxonomic evaluations. These results enhance our understanding of gene rearrangements and evolutionary dynamics in Scolopendromorpha, offering critical insights into their phylogenetic relationships.