Exploring Monacha species from the island of Corfu (NW Greece) by an integrative approach: new insights on M. claustralis (Rossmässler, 1834), M. parumcincta (Rossmässler, 1834) and allied species (Gastropoda, Eupulmonata, Hygromiidae)

By

Manganelli, Giuseppe
Pieńkowska, Joanna R.
Barbato, Debora
Benocci, Andrea
Sosnowska, Katarzyna
Anderson, Roy
Giusti, Folco
Lesicki, Andrzej 1950-

Type

Article

Date of Publication

2025-8-28

Original Publication

ZooKeys

Volume

1250

Pages

201--242

Subjects

allometry , genitalia , LDA ratio extractor , morphometry , nucleotide sequences , PCA ratio spectrum , phylogeography , shell

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.1250.159585

 

Abstract

The Greek island of Corfu (Kérkyra) is considered the type locality of two Monacha species described in 1834 by Rossmässler, namely Monacha claustralis and M. parumcincta. In this work, Corfu populations of these species were investigated by an integrative approach including analysis of morphological features of shell and distal genitalia as well as molecular features of selected mitochondrial and nuclear gene fragments to establish the relationships between Corfu M. claustralis and M. cartusiana as well as between Corfu and Italian M. parumcincta. Shell features did not differentiate the pairs analysed, i.e. Monacha claustralis vs M. cartusiana and Corfu vs Italian M. parumcincta, whereas features of distal genitalia structure and nucleotide sequences of mitochondrial genes (COI and 16SrDNA) distinguished them significantly. Nuclear gene sequences (ITS2 flanked with 5.8S and 28SrDNA fragments) also differentiated between Corfu and Italian M. parumcincta. It is therefore postulated that these two pairs are composed of four separate species: M. claustralis, M. cartusiana, Corfu M. parumcincta, and Italian M. parumcincta, which are distinct from each other and from the other species of the genus Monacha used here for comparison (the six lineages of M. cantiana s.l. and M. pantanellii).