A genome survey of Tetrix japonica (Insecta, Orthoptera) reveals a comparatively small Tetrigidae genome

By

Li, Xuejuan
Chen, Yanpu
Liu, Yuxin
Lin, Li-Liang

Type

Article

Date of Publication

2025-10-1

Original Publication

ZooKeys

Volume

1254

Pages

191--205

Subjects

genome size , high-throughput sequencing , high-throughput sequencing , pygmy grasshopper , repetitive sequence

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.1254.158678

 

Abstract

The pygmy grasshopper, Tetrix japonica, is a common insect widely distributed in eastern Asia. To further investigate its genome characteristics, high-throughput sequencing was used to obtain genome survey data based on Illumina platforms. Analysis of the results showed that the genome size of T. japonica was 1.51 Gb based on K-mer analysis (K-mer = 23), while the size inferred by the flow cytometry (FCM) was 1.94 Gb. The percentage of repetitive sequence was 51.7%, with LINE being the most abundant element (15.46%) and SINE the least abundant element (0.19%). The genome size of T. japonica was relatively smaller compared to other orthopteran species, suggesting a fewer repetitive sequences. These genome survey data can be useful for genetic analysis and phylogenetic evolution of T. japonica and have potential utility in genomic and biological studies of orthopteran species.