Rodentia is the most widely distributed, diverse, and numerous order of the class Mammalia. Nevertheless, rodents are poorly studied in terms of their conservation compared to other mammalian orders. Chile has one of the highest rates of extinction risk in the world for mammals (20%), where rodents have the highest risk (32%). The data of threatened rodent species is not comprehensive, as many species are still classified as data deficient. This lack of information could mean that the actual number of threatened species is higher than currently recognized. Using different databases, the biogeography, conservation status, ecological roles of rodent species in Chile are updated and described, and their potential zoonotic implication discussed. Results revealed that rodent species richness is highest in the northern and central-southern regions of Chile, where fewer protected areas exist, suggesting an inefficient role of public protected areas for the conservation of rodents and potentially other taxa. The conservation classification by the Chilean government did not match the conservation status from international classifications, revealing poor information for several species at national level. Functional traits of the species studied suggest that rodents are good predictors of ecosystem health due to their rapid life cycles and wide distribution, although distribution was predictive for only some species. Our results indicated that better information on the distribution and rodent species richness provide opportunities to understand complex rodent-borne diseases such as hantavirus. This study validates the use of rodents as indicators to assess ecosystem health and design effective biodiversity conservation plans.