Application of RAPD markers for Cuscuta species identification and biodiversity

By

Teofanova, Denitsa
Pachedjieva, Kalina
Tosheva, Anita
Marinova, Bianka
Savov, Stefan
Savov, Martin
Zagorcheva, Tzvetelina
Zagorchev, Lyuben

Type

Article

Date of Publication

2025-10-14

Original Publication

PhytoKeys

Volume

265

Pages

1--12

Subjects

Dodder , genetic diversity , molecular markers , Parasitic plants , RAPD markers

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers [2010]-

Identifiers

DOI: https://doi.org/10.3897/phytokeys.265.152696

 

Abstract

Cuscuta species (dodders) are parasitic plants that harm both native and cultivated flora, presenting significant ecological and economic challenges. This study employs random amplified polymorphic DNA (RAPD) markers to evaluate genetic variation among 70 specimens collected from various localities across Bulgaria. The findings reveal notable differences in RAPD profiles between species, indicating that RAPD markers are effective for species identification, particularly when reproductive organs are absent. While C. campestris exhibited a relatively uniform genetic profile across different populations, native species showed greater genetic heterogeneity, likely due to their development in diverse habitats. Geographical patterns were also observed in the genetic clustering of C. campestris, although seed dispersal mechanisms appeared to play a larger role in shaping genetic diversity than geographic distribution alone. This research highlights the potential of RAPD markers for improving species identification and for understanding the genetic dynamics of Cuscuta populations, which is crucial for managing their spread and minimizing their agricultural impact.