<br> Based on morphological, chemical and phylogenetic analyses, three species of<br> Ochrolechia<br> collected from Guizhou, China, are described as new to science and named<br> Ochrolechia guizhouensis<br> ,<br> O. kuankuoshuiensis<br> and<br> O. leigongshanensis<br> . Phylogenetic trees, based on ITS and mtSSU loci, were constructed using Maximum Likelihood analysis (ML) and Bayesian Inference (BI) methods.<br> Ochrolechia guizhouensis<br> and<br> O. akagiensis<br> are positioned close to each other in the phylogenetic tree.<br> Ochrolechia guizhouensis<br> is morphologically characterised by its apothecia which feature rugose to rosulate, epruinose discs resembling a floral structure. Chemically, the apothecia contain gyrophoric acid, lecanoric acid and lichesterinic acid, while the thallus contains only gyrophoric acid and lecanoric acid. Phylogenetic analysis reveals that<br> Ochrolechia kuankuoshuiensis<br> is closely related to<br> O. parellula<br> and is distinguished by its light yellow, epruinose, deeply rugose apothecial discs, the production of gyrophoric acid and lecanoric acid and the largest ascospores known in the genus.<br> Ochrolechia leigongshanensis<br> forms an isolated phylogenetic branch which is characterised by a soraliate thallus and apothecia with a ring of smooth, salmon-pink tissue on the inner margin. The discs are epruinose to lightly pruinose and plane. The thallus contains gyrophoric acid, lecanoric acid and a trace of atranorin, while the apothecia contain only gyrophoric acid and lecanoric acid. Additionally,<br> O. subrosella<br> and<br> O. longispora<br> were successfully sequenced for the first time and represent the first records from Guizhou Province. We found one specimen, which phylogenetically groups with<br> O. trochophora<br> , but is characterised by the presence of soralia, indicating that<br> O. trochophora<br> might be morphologically more variable than previously thought. Colour photographs are provided for all the above-mentioned species.<br>
