<br> Aims<br> : In Southern Europe, grassland phytosociologists typically do not record non-vascular plant taxa and focus either on annual or perennial vascular plants even when both groups co-occur spatially. By contrast, researchers from temperate and northern Europe typically aim to record the entire community. We thus asked how such a comprehensive sampling approach would affect vegetation classification and biodiversity analyses when applied to Mediterranean dry grasslands.<br> Study area<br> : Island of Sicily, Italy.<br> Methods<br> : We sampled homogeneous stands of dry grasslands of different soil types and from sea level to 1200 m a.s.l. with nested-plot series and additional 10-m<br> 2<br> plots, each with a complete list of all terricolous vascular plant, bryophyte and lichen taxa. For the 67 10-m<br> 2<br> plots we additionally estimated the cover values of the species and determined structural, topographic and soil variables. We subjected these 67 plots to TWINSPAN and determined diagnostic taxa for the clusters at two hierarchical levels.<br> Results<br> : The mean total species richness increased from 2.1 in 0.0001 m<br> 2<br> to 60.6 in 100 m<br> 2<br> with 15% non-vascular plant taxa on average. The four main TWINSPAN clusters corresponded to base-rich coastal sands (mainly<br> Ammophiletea<br> ), acidic inland sands (mainly<br> Poetea bulbosae<br> ), nutrient-rich loamy soils (mainly<br> Chenopodietea<br> ) and alkaline clayish soils (mainly<br> Lygeo-Stipetea<br> ). Each cluster contained annual and perennial plants and mostly also non-vascular plant taxa, both in the species combination and the diagnostic species.<br> Conclusions<br> : Alpha and beta diversity of Mediterranean grasslands is higher than previously thought. While our numerical analyses yielded well characterised and ecologically meaningful units, there was no separation of annual and perennial grassland types at the higher level. We thus recommend recording all vascular plant species occurring in the plot also in Mediterranean regions, so as to allow straightforward comparisons with the rest of Europe in biodiversity studies.<br> <br> <br> Taxonomic reference<br> : Euro+Med (2025) for vascular plants and bryophytes (with very few exceptions given in the Methods), ITALIC 8.0 (Nimis et al. 2025) for lichens.<br> <br> <br> Syntaxonomic references<br> : Mucina et al. (2016) for alliances, orders and classes, except for the<br> Ammophiletea<br> where we adopt the European revision by Marcenò et al. (2024), while those for associations are provided in the syntaxonomic overview.<br> <br> <br> Abbreviations<br> : ANOVA = analysis of variance; CSR = competitive/stress-tolerant/ruderal; DCA = detrended correspondence analysis; EDGG = Eurasian Dry Grassland Group; EIVE = Ecological Indicator Values for Europe 1.0; NMDS = non-metric multidimensional scaling; TWINSPAN = two-way indicator species analysis.<br>
