High mitochondrial DNA sequence divergence in New Guinea Carabdytes stream beetles and the taxonomist’s dilemma when other evidence is kind of subtle… (and collecting localities are far far away)

By

Skale, Andre
Tänzler, Rene
Hendrich, Lars
Balke, Michael

Type

Article

Date of Publication

2012

Original Publication

ZooKeys

Volume

247

Pages

31-43

Subjects

Coleoptera , cryptic species , DNA barcoding , DNA sequencing , integrative taxonomy

Contributed by

Pensoft Publishers

Language

English

Published by

Sofia, Bulgaria Pensoft Publishers

Identifiers

DOI: https://doi.org/10.3897/zookeys.247.3812

 

Abstract

Carabdytes upin tindige ssp. n. is described from the Arfak Mountains, Bird’s Head, Indonesian Papua. It is morphologically very similar to Carabdytes upin upin Balke et al., 1992, known from eastern Indonesian Papua eastward to the western limits of the Papuan Peninsula of Papua New Guinea. For 726 bp at the 3’ end of the mitochondrial cox1 gene, the subspecies differ by 8.1–9.2% uncorrected p-distance. However, we also document considerable cox1 divergence within Carabdytes upin upin. We find few diagnostic positions in the nuclear genes argenine kinase as well as elongation factor 1 alpha that suggest there are indeed two isolated groups of Carabdytes, but evidence in elongation factor 1 alpha is not unambiguous. We decided to highlight this phenomenon of ambiguous evidence for ongoing/just attained speciation by describing a subspecies. We argue that such cases are actually common once mitochondrial sequence data are routinely added to the taxonomist’s toolkit, and sometimes simply adding data from few nuclear genes will not suffice the solve taxonomic riddles. Here, detailed population genetic investigations would be required – for which sufficient numbers of specimens from a sufficiently wide geographical sampling might be nearly impossible to acquire.